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Tnf alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (tnf) superfamily Elisa, western blot, functional assay, immunoprecipitation, immunohistochemistry, flow cytometry and. This cytokine is mainly secreted by macrophage and bind to its receptors, tnfrsf1a/tnfr1 and tnfrsf1b/tnfbr.
It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). These protein have been validated for use in the following applications Tnf alpha binds to its receptors, mainly tnfr1 and tnfr2, and then transmits molecular signals for biological functions such as inflammation and cell death [2].
Cell number was quantitatively assessed by prestoblue® cell viability reagent.
Analyze your elisa data using the rtnf alpha ab9642 protein to generate and plot the standard curve Check out our elisa protocol for more information here. Tnf alpha recombinant proteins are available for human, mouse, cynomolgus monkey, rat and rhesus monkey
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